Somatic Embryogenesis Laboratory


Officially, somatic embryogenesis laboratory is managed by the Indonesian Agency for Agricultural Research and Development. However, daily activities has been entrusted to the Indonesian Centre for Estate Crops Research and Development  (ICECRD). Aimed of the laboratory is to produce uniform superior agricultural seeds in a relatively short time and have the same properties as the parent.


To support the vision of the IAARD become World’s Best R & D Institute in Southeast Asia capable of producing agricultural innovation according to user needs. Through this vision the Laboratory is expected able to generate highly competitive seed multiplication technology


To produce strategic technologies through the improvement and multiplication of plant materials and increase the efficient use of resources to provide value-added of highly competitive farm products.


Programs and activities conducted at the Laboratory is doing plant propagation through tissue culture of various strategic agricultural commodities. For estate crops are  Palm Oil, Cocoa, Coffee, Rubber, Sugar, Bio farmaka, Palma, as well as fiber and sweeteners. For food crops are rice, and beans, while for Hortikultuta are fruits, flowers, and vegetables. Other commodities are Teak and other


• Shelf culture capacity of 40,000 bottles

• Laminar Air Flow: 15 units @ 2 people

• Orbital Shaker

• Bioreactor System: Temporary immersionn System 10 units

• Digital Automatic Autoclave: 2 units of 110 liters and 2 units of 50 liters

• Drying Oven: 4 unit consists of 2 units of 256 liter and 2 units of 53 liter

• Distilling Unit: 2 unit capacity of 2 liters/hour

• Analitical balance: 2 units

• Bench top Balance: 2 units

• Hot Plate Magnetic Stirer: 2 Units

• Magnetic Stirer: 2 units

• Digital pH meter: 2 units

• Pump Drive / Dispenser: 1 unit

• Glass door Refregerator: 2 units

• Refrigerator 2 doors: 2 units

• Rack storage media: 8 units

• Stereo microscope and digital camera: 1 unit each


1) The culture of plants in a bottle,

2) Plant the result of acclimatization,

3) Tissue culture materials,

4) Plants that are ready to sell


• Tool sterelisation and glassware dissection

Medium acidity measurement

• Consultation and plant culture

• Explants preparation

• Explants sterelisation

• Conditioning the sterile culture room

• Compounding simulation of commercial tissue culture media

• Preparation of tissue culture instant media

• Sub-culture (the plant moved to other media)

• Making mother liquor macro salts, micro salts, vitamins, and plant growth regulators

• Preparation of composition media for plants regeneration

• Regeneration of plants through organogenesis and somatic embryogenesis

• Preparation of media for plant tissue culture (initiation, multiplication, and rooting)

• How to overcome the factors that hinder the success of the regeneration process

• How to overcome somaclonal variability in the process of propagation in vitro i.e.

contamination problem, vitrification culture, leaf fall, dormancy, and rooting

• Isolation of explants and sterelisation

• Investment in growth media

• Sub-culture in vitro shoot and root induction

• Acclimatization


Based on the Decree of the Head of ICECRD Number: 848.1/Kpts/KP.320/I.4/05/2011 dated May 31, 2011 management of the laboratory is as follows:


1 Dr. Ir. M Syakir ICECRD Peak Manager
2 Dr. Ir. Karden Mulya ICABGR Peak Deputy Manager
3 Dr. Ir. Ika Mariska ICABGR Technical Manager
4 Dr. Ir. Rubiyo Puslitbangbun Marketing Manager
5 Dra. Endang Hadipoentyanti, MS Balittro Quality Manager
6 Drs. Deden Sukmajaya, Msi ICABGR General Manager
7 Iim Rochimat, SP ICECRD Administration Manager


ICECRD         : Indonesian Centre for Estate Crops Research and Development

ICABGR         : Indonesian Center for Agricultural Biotechnology & Genetic Resources

IMACRI         : Indonesian Medicinal and Aromatic Crops Research Institute

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