Genetic Diversity Analysis of The Cameroon-Originated Oil Palm Accessions Assessed With SSR Markers

Inovasi Teknologi

ESTATE CROPS NEWS – Genetic diversity of the Indonesian oil palm collection is very low. To improve their genetic variability, exploration from the oil palm center of origins has been done in Kamerun.

The objectives of this study were to determine genetic and polymorphism level of the SSR markers Cameroonoriginated oil palm accessions. Genetic materials used were 49 Cameroonoriginated oil palm accessions collected at Sijunjung Oil Palm Germplam Collection Station, West Sumatera. Genomic DNA was isolated using a protocol for isolating DNA from leaves rich with latex. DNA was analyzed using 20 SSR markers. A dendogram was constructed using the Unweighted Pair Group Method Arithmetic (UPGMA) method through the Numerical Taxonomy and Multivariate Analysis System software (NTSYS-pc) version 2.1-pc.

Results showed that the polimorfisme information content (PIC) values of the SSR markers used was high, 0.80 (range from 0.63-0.91). The average number of the SSR alleles detected was also high, 8.75 alleles (range from 4-15 alleles per SSR locus).

Phylogenetic analysis of the 49 oil palm accessions resulted genetic diversity of 12.5-54.72% (genetic similarity of 55.28-87.50%). At genetic diversity 54.72%, the 49 accessions were divided into seven clusters, each consisted of 9, 28, 4, 2, 1, 2, and 3 accesions, respectively. Accessions with high genetic diversity and located at different clusters may be useful as parent candidates in the future oil palm breeding programs. (/I Made Tasma, dan Sekar Arumsari)

Key words: Elaeis guineensis Jacq., genetic diversity, germplasm, SSR markers.

Download full text: perkebunan_Jurnal-I-Made-Tasma-5.pdf

Source: Jurnal Littri ICECRD Vol. 19 No. 4, 2013

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