Screening of Cotton Lines (Gossypium hirsutum L.) Tolerance to Drought at Germination Stage with PEG-6000

Artikel Kapas Berita Perkebunan

ESTATE CROPS NEWS – Cotton production areas in Indonesia are arable land with lack of water availability. The aim of this study was to obtain cotton lines tolerant to drought using PEG-6000 at germination stage. The experiment was conducted at the Seed Testing Laboratory in Indonesian Sweetener and Fiber Crops Research Institute, from April to June 2012.

Treatments were arranged in a Split Plots design with two replications. The main plot was PEG-6000 (-3 bar) and without PEG-6000 (water), while the subplot was 13 cotton lines and Kanesia 14 variety. Seed cotton was treated with 80% Mancozeb fungicide dose of 2g/kg seed before sowing. Parameters observed were germination percentage, shoot and root length, shoot and root weight, ratio of root/shoot length, and drought susceptibility index. Seedling was counted as normal if its length more than 0.5 cm. PEG-6000 treatment gives very significant effect on the decline on seed germination, shoot and root length, shoot and root weight. Otherwise, ratio of root/shoot length was higher in the PEG-6000 than without PEG-6000 treatment. Response of cotton lines to drought which calculated with a drought susceptibility index were different among parameter observed. Mean drought susceptibility index of all parameters showed that none of cotton line tolerant to drought (S < 0,50) was achieved from the study. There were eight moderately drought-resistant lines (0, 50 < S < 1,0) achieved namely 03002/12, 03006/1, 03008/7, 03008/24, 03012/17, 03014/12, 03017/13, and 03017/15 which more resistance than Kanesia 14. (\Siwi Sumartini, Emy Sulistyowati, Sri Mulyani, and Abdurrakhman/Balittas)

Source : Jurnal Littri Puslitbang Perkebunan Vol. 19 No. 3, 2013

Download Fulltext: perkebunan_jurnal-littri_Vol19313_5.-Siwi-Sumartini1.pdf

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