Mutant Planlet Formation of Sugarcane Tolerant Sodium Chloride Through In Vitro Selection and Mutation

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ICECRD PUBLICATIONS – The needs of sugarcane continue to increase so extensification of farming is needed to meet the demand. However, the available land is the sub-optimal land such as saline land. For this reason, salinity tolerant varieties are needed. To used create sugarcane varieties that are tolerant to salinity stress induction mutation technology using gamma rays combined with in vitro selection using NaCl can be used. The purpose of this study was to obtain sugarcane mutant planlets which were tolerant to the salinity resulted from the induction mutations and in vitro selection. The plant material used in this study were PS862 and PSJT941 sugarcane varieties. The environmental design used in this study was a completely randomized design. This study consisted of four main stages of activity namely (1) mutation induction using gamma ray irradiation (5, 10, 15 20, 25.30 and 35 Gy) and in vitro selection on media containing NaCl; (2) bud regeneration in MS medium + BA 3 mg / l + Zeatin 0.3 mg / l + Proline 100 mg / l for sugarcane callus PS862 and MS varieties + BA 3 mg / l + Zeatin 0.1 mg / l + Proline 100 mg / l for sugarcane callus PSJT941 and (3) root induction on MS + IBA 1 mg / l. The results of this study were 122 mutant plantlets originating from the PS862 variety and 66 mutant planlets originating from PSJT941 which were tolerant to NaCl salt stress. The mutants obtained were salinity tolerant because they were able to grow on media containing NaCl. To produce a population that is salinity tolerant, it is necessary to test it in a greenhouse and in the field that is gripped by salinity.

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