Induced Mutation Using Colchicine and in Vitro Selection Using Polyethylene Glycol for Drought-Tolerant Sugarcane

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ESTATE CROPS INFO – Creating of varieties can be done through mutation breeding at the cellular level, combined with in vitro selection. This research was conducted at the UPBUP from January until December 2017 to find out colchicine concentration and treatment duration which effectively produced tolerant mutant through in vitro drought selection using polyethylene glycol (PEG). The study consists of two stages. The first was mutation induction on sugarcane calli using colchicine, which was arranged factorially with a completely randomized environment design. The first factor was varieties (BL, PS 862, and PSJT 941), the second was colchicine concentration (0,01,0,0 and 0,05%), and the third was colchicine duration treatment (1 and 3 days). Observations were made on the percentage of callus survival. The second stage was in vitro selection of droughts using a PEG 6000, which was arranged factorially with a complete randomized design. The first factor was the concentration of colchicine (0, 0.01, 0.03, and 0.05%), the second was the colchicine duration treatment (1 and 3 days), and the third was PEG concentration (0, 10 and 20%). Selection was done for 4 weeks. Percentage of live callus, regenerated callus, number and height of shoots were observed as a selected criteria. Colchicine treatment in the 0.01 – 0.05% for 3 days on PS 862 and 0.01 – 0.03% for 3 days on PSJT 941 callus resulted mutant passing in vitro drought selection at 10% PEG concentration level. Mutant selection will be continued through in vivo. The optimum mutation treatment for BL has not been obtained.

Keywords: chemical mutagen, colchicine, mutation, selection agent, PEG 6000

Related link : Induced Mutation Using Colchicine and in Vitro Selection Using Polyethylene Glycol for Drought-Tolerant Sugarcane

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