Endophytic Bacteria from Purwoceng as Steroid and Antipatogenic Compounds Producers
ESTATE CROPS INFO – Purwoceng (Pimpinella pruatjan Molk.) is a commercial medicinal plant from Indonesia that is useful as an aphrodisiac, diuretic, and tonic. Purwoceng are known to produce some secondary metabolite compounds of phytosterol (stigmasterol, sitosterol, bergapten, ergosterol, amirin, and vitamin E). The quantity of phytosterol compounds isolated from this plant is very small, but have many benefits. Bioactive compounds that are nutritious in a plant was also produced by endophytic microbes. It gives the opportunity to produce sterol compounds using endophytic microbial culture found in purwoceng. The objectives of this study were to screen and characterize endophytic bacteria from purwoceng that are antagonistic to a number of pathogenic microbes (Enteropatogenic Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Pseudomonas pseudomallei, and Candida albicans), and produce sterol compounds. The paper disc diffusion method is used for antipatogenic tests and Thin Layer Chromatography analysis for analysis of sterol compounds. Sterol compounds obtained from endophytic bacterial isolates Endo PWC I.GP-1. Some isolates were able to prevent the growth of pathogens (Staphylococcus aureus, Listeria monocytogenes, and Enteropathogenic Escherichia coli (EPEC), Endo PWC I.D2, Aktino PWC GP-4, Endo PWC GP-2, Endo PWC D-1, and Endo PWC I.GP-1. Endo PWC I.GP-1 endophytic isolate which capable of producing sterol compounds and such antibacterial metabolites is identified as Corynebacterium sp.
Keywords: Purwoceng, endophytic bacteria, sterols, antipatogenic